Asian Journal of Research and Reports in Endocrinology

Comparative Analysis of the Anti-diabetic Effects of Cocoyam, Soya Bean & Bambara Groundnut Flour Fed Differently to Streptozotocin (STZ)-Induced Diabetic Rats

Uro-Chukwu, H.C *

Department of Biochemistry, Coal City University, Enugu, Nigeria, Department of Community Medicine, Ebonyi State University, Abakaliki, Nigeria and Institute of Nutrition, Nutraceuticals, Public Health Research & Development, Nigeria.

Ezekwe, A.S

Department of Medical Biochemistry, Rivers State University, Nkpolu, Port Harcourt, Nigeria.

Roberts, F

Department of Medical Biochemistry, Rivers State University, Nkpolu, Port Harcourt, Nigeria.

Okari, K.A

Department of Medical Biochemistry, Rivers State University, Nkpolu, Port Harcourt, Nigeria.

Uro-Chukwu, F.C.U

Institute of Nutrition, Nutraceuticals, Public Health Research & Development, Nigeria.

*Author to whom correspondence should be addressed.

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Abstract

Background: Diabetes mellitus (DM) with the common type, being, Type 2 Diabetes Mellitus, affects a vast population globally especially within the age range of 20 – 79 years, with the need for cure being prioritized by the World Health Organization, who champions the use of cheaper treatment strategies as a result. Food-based approach including use of medical plants have been canvassed as a preventive and Public Health remedy for the management of DM. Plants foods like Cocoyam (CYN), soya bean (SB), and Bambara groundnut (BGN) have been reported to have health benefits, and hence the need in this study to evaluate and compare the anti-diabetic effects in diabetic rats.

Methodology: Cocoyam (CYN), soya bean (SB), and Bambara groundnut (BGN) were processed into fine flour, pelletized, oven-dried at 60°C, and stored in airtight container for future use. Insulin resistance and Type 2 Diabetes were induced with low fructose diet and intraperitoneal injection of streptozotocin respectively on fifty-eight male albino rats with weight range of 134 to 247 g. The intervention formulations were administered for 28 days, while blood samples were collected from the killed animals for random and fasting blood glucose levels.

Results and Discussion: The reports from the study revealed a mean random blood glucose (RBG) levels that changed over the time trend (F=13.963, p<0.001) with a similar group pattern in weeks 1, 2, 3 and 4 (F=79.106, p<0.01) (F=76.755, p<0.001). The mean value of the random blood glucose in the rat group fed on Bambara groundnut (BGN) was better than that of the SB group, which was better than that of the group given CYN (BGN>SB>CYN). When the intervention groups were compared with the control group, the CYN and SB groups had better anti-diabetic outcomes.

Conclusion: Plant foods, Bambara groundnut, Soya bean and Cocoyam possess anti-diabetic properties but the potency of the hypoglycemic effects when compared showed that Bambara groundnut was more effective in glycemic control in diabetic rats and hence can be used as an additional therapeutic adjunct in the management of Type 2 diabetes mellitus.

Keywords: Diabetic rats, anti-hyperglycemia, cocoyam, bambara groundnut, soya bean

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References

Sun H, Saeedi P, Karuranga S, Pinkepank M, Ogurtsova K, Duncan BB, Stein C, Basit A, Chan JCN, Mbanya JC, Pavkov ME, Ramachandaran A, Wild SH, James S, Herman WH, Zhang P, Bommer C, Kuo S, Boyko EJ, Maqliano DJ. IDF Diabetes Atlas: Global, Regional and Country-level diabetes prevalence estimates for 2021 and projections for 2045. Diabetes Research and Clinical Practice. 2022;183:109119. Available:https://doi.org/10.1016/j.diabres.2021.109119

Van Belle TL, Coppieters KT, Von Herrath MG. Type 1 diabetes: Etiology, immunologyand therapeutic strategies. Physiological Reviews. 2011;91:1.

Lin X, Xu Y, Pan X, et al. Global, regional, and national burden and trend of diabetes in 195 countries and territories: An analysis from 1990 to 2025. Sci Rep. 2020;10:14790.

Kirigia JM, Sambo HB, Sambo LG and Barry SP. Economic burden of diabetes mellitus in the WHO African region. BMC International Health and Human Rights. 2009;9:6.

Sobngwi E, Mauvais-Jarvis F, Vexiau P, Mbanya JC, Gautier JF. Diabetes in Africans. Epidemiology and clinical specificities. Diabetes Metab. 2001;27(6): 628-634.

Piero MN. Hypoglycemic effects of some Kenyan plants traditionally used in management of diabetes mellitus in eastern province, thesis, Kenyatta University; 2006.

Wylie-Rosett J, Delahanty LM. Nutrition in the Prevention and Treatment of Disease (Fourth Edition). 2017;691-707. Available:https://doi.org/10.1016/B978-0-12-802928-2.00031-X

Forouhi GN, Misra A, Mohan V, Taylor R, Yancy W. Dietary and nutritional approaches for prevention and management of type 2 Diabetes, Science and Politics of Nutrition, BMJ. 2018;361 Available:https://doi.org/10.1136/bmj.k2234

Piero MN, Njagi JM, Kibiti CM, Ngeranwa JJN, Njagi ENM, Miriti PM. The Role of Vitamins and Mineral Elements in Management of Type 2 Diabetes Mellitus: A Review South As. J. Biol. Sci. 2012; 2(Supp.1):107 –115.

Arise AK, Amonsou EO, Ijabadeniyi OA. Influence of extraction methods on functional properties of protein concentrates prepared from South African Bambara groundnut landraces. International. Journal of Food Science and. Technology. 2015;50:1095–1101. Available:https,//doi.org/10.1111/ijfs.12746.

Oyeyinka AT, Pillay K, Siwela M. Full title- In vitro digestibility, amino acid profile and antioxidant activity of cooked Bambara groundnut grain. Food Bio sci. 2019;31: 100428. DOI: 10.1016/j.fbio.2019.100428

Salawu. Comparative study of the antioxidant activities of methanolic extract and simulated gastrointestinal enzyme digest of Bambara nut (Vigna subterranean) FUTA J. Res. Sci. 2016;1: 107-120.

Barbieri R, Coppo E, Marchese A, Daglia M, Sobarz-Sánchez E, Nabavi SF, Nabavi SM. Phytochemicals for human disease: An update on plant-derived compounds antibacterial activity. Microbiology Research. 2017;196:44-68.

Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of medicinal plants. Journal of Pharmacognosy and Phytochemistry. 2013;1(6):168–182.

Iwai K, Kim M, Onodera A, Matsue H. α-Glucosidase inhibitory and antihyperglycemic effects of polyphenols in the fruit of viburnum dilatatum Thunb. Journal of Agricultural and Food Chemistry. 2006;54(13):4588-4592.

Ruzaidi A, Abbe M, Amin L, Nawalyah AG, Muhajir H, Pauliena MB, Muskinah MS. Hypoglycemic properties of Malaysia cocoa (Theobromacacao) polyphenols-rich extract. Int Food Res. J. 2008;15:305- 312.

Merida LA, Mattos EB, Correa AC, Pereira PR, Paschoalin VM, Pinho MF, Vericimo MA. Tarin stimulates granulocyte growth in bone marrow cell cultures and minimizes immunosuppression by cyclo-phosphamide in mice. Plos One. 2018; 13:e0206240. DOI: 10.1371/journal.pone.0206240

Chukwuma CI, Islam MS, Amonsou EO. A comparative study on the physicochemical, anti-oxidative, anti-hyperglycemic and anti-lipidemic properties of amadumbe (Colocasia esculenta) and okra (Abelmoschus esculentus) mucilage. J. Food Biochem. 2018;42:e12601. DOI: 10.1111/jfbc.12601

Prabhakar PK, Doble M. A target based therapeutic approach towards diabetes mellitus using medicinal plants. Current Diabetes Reviews. Bentham Science Publishers Ltd. 2008;291-308.

Kumawat NS, Chaudhari SP, Wani NS, Deshmukh TA, Patil VR. Antidiabetic activity of ethanol extract of Colocasia esculenta leaves in alloxan induced diabetic rats. International Journal of Pharmaceutical Technique Research. 2010;2:1246–1249.

Eleazu CO, Iroaganachi M, Eleazu KC. Ameliorative potentials of cocoyam (Colocasia esculenta L.) and unripe plantain (Musa paradisiaca L.) on the relative tissue weights of streptozotocin-induced diabetic rats. J Diabetes Res. 2013;1–8. Available:https://doi.org/10.1155/2013/160964

Carvalho AW, Silva CO, Dantas MIS, Natal DIG, Ribeiro SMR, Costa NMB, Martino HSD. The use hull soybean flour of heat-treated grains does not affect Iron bioavailability in rats. Archivos Latinoamericanos de Nutrición. 2011; 61(2):135-42.

Martino HSD, Martin BR, Weaver CM, Bressan J, Esteves EA, Costa NMB. Zinc and iron bioavailability of genetically modified soybeans in rats. Journal of Food Science. 2007;72(9):689-695

NRC (National Research Council). Guide for the care and use of laboratory Animals. Bethesda (MD): National Institute of Health. 1985;8523.

Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clinical Pharmacology. 2016;7(2):27-31. DOI: 10.4103/0976-0105.177703

Pneu-Dart. Dosage Calculation; 2023. Available:https://www.pneudart.com. Accessed 14th April 2024

Nnadi NN, Ezekwesili CN, Ezeigwe OC. Effects of formulated unripe plantain and millet dietary feeds in Alloxan-induced diabetic albino rats. International Journal of Innovative Research and Advanced Studies (IJIRAS). 2022;9(6).

Azeez TA. The pattern of antidiabetic drugs and glycemic control among type 2 diabetes patients in an endocrinology clinic in Lagos, Nigeria; 2023. Available:https,//doi.org/10.110//2023.06.25.23291774

Yamamoto-Honda R, Takahashi Y, Mori Y, Yamashita S, Yoshida Y, Kawazu S, Iwamoto Y, Kalio H, Yanai H, Mishima S, Handa N, Shimokawa K, Yoshida A, Watanabe H, Ohe K, Shimbo T, Noda M. Changes in antidiabetic drugs prescription and glycemic control Trends in Elderly patients with type 2 diabetes mellitus from 2005 – 2013: An analysis of the National Centre for Diabetes Database. Internal Medicine. 2018;57(9):1229- 1240.

Garba HA, Mohammed A, Ibrahim MA, Shuaibu MN. Effect of lemongrass (Cymbopogon citratus Stapf) tea in a type 2 diabetes rat model. Clinical Phytoscience. 2020;6:19.

Guo Y, Fu W, Xin Y, Bai J, Peng H, Fu L, Liu J, Li L, Ma Y, Jiang H. Antidiabetic and antiobesity effects of artemether in db/db mice. Bio Med Research International. 2018;8(6):39-52.

Furukawa A, Arita T, Fukuzaki T, Mori M, Honda T, Satoh S, Matsui Y, Wakabayashi K, Hayashi S, Nakamura KJ. Synthesis and biological evaluation of novel (−) cercosporamide derivatives as potent selective PPARγ modulators. Bioorganic and Medicinal Chemistry Letters. 2012; 54:522-533.

Oršolić N, Sirovina D, Odeh D, Gajski G, Balta V, Šver L, Jembrek MJ. Efficacy of caffeic acid on diabetes and its complications in the mouse. Molecules. 2021;26(11):3262. DOI: 10.3390/molecules26113262

Oboh G, Ogunbadejo MD, Ogunsuyi OB, Oyeleye SI. Can gallic acid potentiate the antihyperglycemic effect of acarbose and metformin? Evidence from streptoztocin-induced diabetic rat model. Archives of Physiology and Biochemistry; 2020. Available:https://doi.org/10.108/13813455.2020.1716014.

Huang DW, Chang WC, Wu JS, Shih RW, Shen SC. Gallic acid ameliorates hyperglycemia and improves hepatic carbohydrate metabolism in rats fed a high-fructose diet. Nutrition Research. 2016;36(2):150–60.

Zafar M, Naqvi SMH. Effects of STZ-induced Diabetes on the relative weights of kidney, liver and pancreas in Albino Rats: A Comparative Study. International Journal of Morphology. 2010;28(1):135-142

Lee SI, Kim JS, Oh SH, Park KY. Anti-hyperglycemic effect of Fomitopsis pinicola extracts in streptozotocin-induced diabetic rats. Journal of Medicinal Food. 2008;11(3):518–524.

Piyachaturawat P, Poprasit J, Glinsukon T, Warichanon C. Gastric mucosal lesions in Streptozotocin-diabetic rats. Cellullar Biology. 1988;12(1):53-63.

Habibuddin M, Daghriri HA, Humaira T, Al-Qahtani MS, Hefzi AA. Antidiabetic effect of alcoholic extrac of Caralluma sinaica L. on streptozotocin-induced diabetic rabbits. Journal of Ethnopharmacology. 2008;117(2):215-20.

Eleazu CO, Eleazu KC, Iroaganachi MA. Effect of cocoyam (Colocasia esculenta), unripe plantain (Musa paradisiaca) or their combination on glycated hemoglobin, lipogenic enzymes, and lipid metabolism of streptozotocin-induced diabetic rats, Pharmaceutical Biology. 2016;54(1):91-97. DOI: 10.3109/13880209.2015.1016181