Evaluation of Selected Biochemical Indices of Diabetic Rats Treated with Methanol Leaf Extract of Andropogon gayanus
Ogwo Elisha Uko *
Department of Human Physiology, Abia State University, Uturu, Abia State, Nigeria.
Ejiofor Dominic Chinedu
Department of Human Physiology, Imo State University, Owerri, Nigeria.
Obioma Elizabeth Nweke
Department of Human Anatomy, Chukwuemeka Odumekwu Ojukwu University, Uli, Nigeria.
Ubani Clement Dimgba
Department of Human Physiology, Abia State University, Uturu, Abia State, Nigeria.
Iheukwumere Barry Chinedu
Department of Human Physiology, Abia State University, Uturu, Abia State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The aim of this study was to evaluate the biochemical changes in diabetic Wistar rats administered methanol leaf extract of Andropogon gayanus. Freshly harvested leaves of A. gayanus were processed into extract. Twenty-five adult male Wistar rats were divided into five groups of five rats per group. Group I was the normal control and was administered 2 mL of distilled water. Group II: was induced diabetes without treatment. Group III: were diabetic rats treated with 100 mg/kg of A. gayanus leaf extract. Group IV: was diabetic rats treated with 200 mg/kg of A. gayanus leaf extract. Group V: was diabetic rats treated with standard drugs. The activities of AST, ALT and ALP in diabetic groups treated with the aforementioned extract were significantly (P<0.05) lower than those reported for the normal control. However, a contrary observation was made on the serum albumin and total protein. Total cholesterol (TC) and Triglycerides as well as Low-Density Lipoprotein reported for groups treated with extract were significantly (P<0.05) higher than those reported for the negative control. However, a contrary observation was made on the High-Density Lipoprotein (HDL). Serum creatinine and urea reported for the negative control were significantly (P<0.05) higher than those reported groups administered leaf extract of Andropogon gayanus. In conclusion, it can be deduced from this study that methanol leaf extract of Andropogon gayanus has influence on the indices evaluated which can be traced to the biological activities of certain active ingredients inherent in them.
Keywords: Protein, serum, diabetes, Andropogon gayanus, albumin, cholesterol, triglycerides, lipoprotein, hyperglycemia
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References
American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2014;37:S81–90.
American Diabetes Association. Microvascular complications and foot care: Standards of medical care in diabetes—2018. Diabetes Care. 2018;41: S105–18.
Akter S, Rahman MM, Abe SK, Sultana P. Prevalence of diabetes and prediabetes and their risk factors among Bangladeshi adults: a nationwide survey. Bull World Health Organ. 2014;92:204-213A.
Zheng Y, Ley SH, Hu FB. Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nat Rev Endocrinol. 2018;14(2):88.
IDF. International Diabetes Federation. IDF Diabetes Atlas — 8th Edition. Diabetes Atlas; 2017.
Available: https ://diabe tesat las.org/resou rces/2017-atlas.html. Accessed March 2019.
Philip R, Mathias M, Kumari SN, Gowda DK, Shetty JK. Evalation of relationship between markers of liver function and the onset of type 2 diabetes. Nitte Univ J Health Sci. 2014;4(2):90.
Corbin WL. Herbs as Medicines. Archives of Internal Medicine. 1998;158:2192-2199.
NIH. NIH Consensus Development Panel on Impotence. JAMA. 1993;270:83-90.
Lorke D. A new approach to practical acute toxicity testing. Archive Toxicology. 1983; 54:275-287.
Burcelin R, Eddouks M, Maury J, Kande J, Assan R, Girard J. Excessive glucose production, rather than Insulin resistance, account for hyperglycemia in recent onset streptozotocin-diabetic rats. Diabetologia. 1995;35:283-290.
Reitman S, Frankel S. Modified reitman and frankel method of AST and ALT estimation. Amer. J.Clin.pathol. 1957;28: 56-63.
Kaplan A, Teng LL. Selected methods of clinical chemistry. In WR Faulkner, Mietes S (Eds.), AACC, Washington. 1982;357-363.
Bartels H, Bohmer M. Clinical chemistry. Acta. 1972;37:193.
H Wieland, D Siedel. Artzl. Lab. 1981;27: 141-144.
George RK. Biochemistry laboratory. Philadelphia; 2009.
DOI:www.jbc.org
Bjorston AR, Crankshaw DP, Morgan DJ, Prideaux PR. Clinical chemistry. Department of surgery, Royal Malbourne Hospital, University of Melbourne, Victoria, 3050; 2007.
Zandam SU, Nazifi AB, Odoma S, Zezi AU. Anti-nociceptive and anti-inflammatory activities of methanol root extract of Andropogon gayanus Kunth (Poaceace) in rodents. Afr J Pharmacol Ther. 2020;9(1): 27-33.
Michael I. Oraebosi, Melford U. Elendu. Hepatoprotective effect of andropogon gayanus against paracetamol- and carbontetrachloride-induced liver toxicity in rats. Tropical Journal of Natural Product Research. Trop J Nat Prod Res. 2021, January;5(1):188-193
Ren J, Fu L, Nile SH, Zhang J, Kai GY. Salvia miltiorrhiza in treating cardiovascular diseases: A review on its pharmacological and clinical applications. Front. Pharmacol. 2019;10: 753.
Ul Islam S, Ahmed MB, Ahsan H, Lee YS. Recent molecular mechanisms and beneficial effects of phytochemicals and plant-based whole foods in reducing LDL-C and preventing cardiovascular disease. Antioxidants. 2021;10:784.
Wolf G, Ziyadeh FN. Cellular and molecular mechanisms of proteinuria in diabetic nephropathy. Nephron Physiol. 2007;106:26–31.
Nephroprotective Potential of a Standardized Extract of Bambusa arundinacea: In Vitro and In Vivo Studies Saba Muzher Iqbal, Liaqat Hussain, Musaddique Hussain, Haseeb Akram, Muhammad Asif, Ayesha Jamshed, Ammara Saleem, and Rida Siddique. This: ACS Omega. 2022;7: 18159−18167.