Effect of Aqueous Leaf Extract of Hamelia patens jacq. on Some Biochemical Parameters in Alloxan- Induced Diabetic Albino Rats
Published: 2022-08-10
Page: 101-109
Issue: 2022 - Volume 5 [Issue 1]
C. Aguwamba
Department of Biochemistry, Cllifford University, Owerrinta, Nigeria.
C. J. Nwaogwugwu *
Department of Biochemistry, Faculty of Biological Sciences, Abia State University, PMB 2000, Uturu, Nigeria.
C. I. Nosiri
Department of Biochemistry, Faculty of Biological Sciences, Abia State University, PMB 2000, Uturu, Nigeria.
M. E. Uche
Department of Biochemistry, Cllifford University, Owerrinta, Nigeria.
C. Aaron
Department of Biochemistry, Cllifford University, Owerrinta, Nigeria.
E. Ike, W.U
Department of Biochemistry, Cllifford University, Owerrinta, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Background: Medicinal plants is as old as man, they contain substances that can be used for therapeutic purposes with less toxicity. A number of these plants, leaf, herb and stem bark has been used in traditional medicine to treat diabetes for many years without sufficient scientific data.
Aim: Effect of aqueous leaf extract of Hamelia patensjacq. on some biochemical parameters in alloxan-induced diabetic albino rats was determined.
Method: The experimental animals (male Albino rats) were divided randomly into six groups of eight animals each. The test groups (2-6) were starved overnight followed by intraperitoneal administration of alloxan at 160mg/kg body weight. Treatment with the plant extract was for fourteen days. Group 1 was the normal control, group 2; diabetic untreated animals, group 3 received the standard drug gliclazide at 58mg/ml, groups 4, 5 and 6 were the extract treated groups at 400mg/kg body weight, 800mg/kg body weight and 1200mg/kg body weight respectively.
Results/Discussion: The mean lethal dose of the plant extract was above 5000mg/kg body weight. Total protein increased significantly (P<0.05) in the extract treated groups while, aspartate aminotransferase (AST), alanintransaminase (ALT), alkaline phosphatase (ALP) had significant decreases (P<0.05) when compared to group 2. Malondialdehyde (MDA), decreased significantly (P<0.05) in the group that received the standard drug alongside the extract treated groups when compared to group 2, while Glutathione (GSH) and Superoxide dismutase had a significant decrease in groups 2 and 3 at (P<0.05) and was non significant in the extract treatedgroups at (P>0.05). The C-peptidesincreased significantly (P<0.05) in groups 4 to 6 when compared to the group 2 that had a higher significant increase (P<0.05).
Conclusion: The results show that aqueous leaf extract of Hamelia patens may possess anti-diabetic,anti-inflammatory, antihepatotoxic, antianemic, antioxidant and antilipidemic properties at the tested doses.
Keywords: Blood glucose, antidiabetic, antioxidant, antilipidemic, antihepatotoxic, Alloxan