Nigella sativa and Ginger Increase GLUT4 and PPARγ in Metabolic Syndrome‐induced Rats
Asian Journal of Research and Reports in Endocrinology,
Background: Increased fructose intake has been linked to epidemiology of insulin resistance, type 2 diabetes mellitus, renal damage and metabolic syndrome (MS). As oxidative stress plays a pivotal role in the pathology of insulin resistance, the present study was conducted to investigate the effects of Nigella Sativa (NS) and ginger, as potent antioxidants on fructose induced MS in rats.
Methods: Male rats were fed with high‐fructose high‐fat fed diet for 8 weeks. By the end of the 8th week, rats were divided into four groups; one was left untreated (normal control) and MS control group treated with saline, MS groups given Nigella sativa (4 ml/kg), and ginger (500 mg/kg) daily for 4 weeks. Markers chosen for assessment included effect on body weight gain, glucose, insulin, adiponectin levels, and lipid profile. Also glucose transporter 4 (GLUT4) content and peroxisome proliferator‐activated receptor‐gamma (PPARγ) protein expressions were estimated.
Results: Nigella sativa and ginger ameliorated some manifestation of MS including increase in body weight, glucose, insulin level and resistance. In addition, both drugs lowered insulin resistance induced hyperlipidemia and increased adiponectin level. Drugs also increased GLUT4 and PPARγ protein expression compared with MS control group.
Conclusion: Nigella sativa and ginger ameliorated parameters of MS. They improve the lipid profile and insulin sensitivity via increased adiponectin, GLUT4 and PPARγ expression.
- Metabolic syndrome
- Nigella sativa
- Insulin resistance
How to Cite
Roberts CK, Hevener AL, Barnard RJ. Metabolic syndrome and insulin resistance: underlying causes and modification by exercise training. Comprehensive Physiology. 2013;3(1):1-58.
Tappy L, Lê KA. Metabolic effects of fructose and the worldwide increase in obesity. Physiological reviews. 2010; 90(1):23-46. Samuel VT, Shulman GI. J Clin Invest. 2016;126:12. Supruniuk E, Mikłosz A, Chabowski A. Front Physiol. 2017;8:923.
Ganji A, Salehi I, Nazari M, Taheri M, Komaki A. Effects of Hypericum scabrum extract on learning and memory and oxidant/antioxidant status in rats fed a long-term high-fat diet. Metabolic Brain Disease. 2017;32(4):1255-1265.
Leong XF, Rais Mustafa M, Jaarin K. Nigella sativa and its protective role in oxidative stress and hypertension. Evidence-Based Complementary and Alternative Medicine; 2013.
Mollazadeh H, Hosseinzadeh H. The protective effect of Nigella sativa against liver injury: a review. Iranian Journal of Basic Medical Sciences. 2014;17(12):958.
Boskabady MH, Keyhanmanesh R, Khameneh S, Doostdar Y, Khakzad MR. Potential immunomodulation effect of the extract of Nigella sativa on ovalbumin sensitized guinea pigs. Journal of Zhejiang University Science B. 2011;12(3):201-209.
El Rabey HA, Al-Seeni MN, Bakhashwain AS. The antidiabetic activity of Nigella sativa and propolis on streptozotocin-induced diabetes and diabetic nephropathy in male rats. Evidence-Based Complementary and Alternative Medicine; 2017.
Eissa FA, Choudhry H, Abdulaal WH, Baothman OA, Zeyadi M, Moselhy SS, Zamzami MA. Possible hypocholesterolemic effect of ginger and rosemary oils in rats. African Journal of Traditional, Complementary and Alternative Medicines. 2017;14(4):188-200.
Marx W, McKavanagh D, McCarthy AL, Bird R, Ried K, Chan A, Isenring L. The effect of ginger (Zingiber officinale) on platelet aggregation: A systematic literature review. PLoS One. 2015;10(10): e0141119.
Akinyemi AJ, Ademiluyi AO, Oboh G. Aqueous extracts of two varieties of ginger (Zingiber officinale) inhibit angiotensin I–converting enzyme, iron (II), and sodium nitroprusside-induced lipid peroxidation in the rat heart in vitro. Journal of Medicinal Food. 2013;16(7):641-646.
Hosseinian S, Roshan NM, Khazaei M, Shahraki S, Mohebbati R, Rad AK. Renoprotective effect of Nigella sativa against cisplatin-induced nephrotoxicity and oxidative stress in rat. Saudi Journal of Kidney Diseases and Transplantation. 2018;29(1):19.
Hassanali Z, Ametaj BN, Field CJ, Proctor, SD, Vine DF. Dietary supplementation of n‐3 PUFA reduces weight gain and improves postprandial lipaemia and the associated inflammatory response in the obese JCR: LA‐cp rat. Diabetes, Obesity and Metabolism. 2010;12(2):139-147.
Sakai C, Ishida M, Ohba H, Yamashita H, Uchida H, Yoshizumi M, Ishida T. Fish oil omega-3 polyunsaturated fatty acids attenuate oxidative stress-induced DNA damage in vascular endothelial cells. PloS One. 2017;12(11):e0187934.
Tanka-Salamon A, Komorowicz E, Szabó L, Tenekedjiev K, Kolev K. Free fatty acids modulate thrombin mediated fibrin generation resulting in less stable clots. PloS One. 2016;11(12):e0167806.
Calvo-Ochoa E, Hernández-Ortega K, Ferrera P, Morimoto S, Arias C. Short-term high-fat-and-fructose feeding produces insulin signaling alterations accompanied by neurite and synaptic reduction and astroglial activation in the rat hippocampus. Journal of Cerebral Blood Flow & Metabolism. 2014;34(6):1001-1008.
Ahmed MA, Hassanein KM. Cardio protective effects of Nigella sativa oil on lead induced cardio toxicity: Anti-inflammatory and antioxidant mechanism. J Physiol Pathophysiol. 2013;4(5):72-80.
Thomson M, Al-Amin ZM, Al-Qattan KK, Shaban LH, Ali M. Anti-diabetic and hypolipidaemic properties of garlic (Allium sativum) in streptozotocin-induced diabetic rats. Int J Diabetes & Metabolism. 2007; 15:108-115.
Van Herck H, Baumans V, Brandt CJWM, Boere HAG, Hesp APM, Van Lith HA, Beynen AC. Blood sampling from the retro-orbital plexus, the saphenous vein and the tail vein in rats: comparative effects on selected behavioural and blood variables. Laboratory Animals. 2001;35(2):131-139.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry. 1972; 18(6):499-502.
Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985;28(7):412-419.
McAuley KA, Williams SM, Mann JI, Walker RJ, Lewis-Barned NJ, Temple LA, Duncan AW. Diagnosing insulin resistance in the general population. Diabetes Care. 2001;24(3):460-464.
Elliott SS, Keim NL, Stern JS, Teff K, Havel PJ. Fructose, weight gain, and the insulin resistance syndrome. Am J Clin Nutr. 2002;76(5):911.
Tunduguru R, Thurmond DC. Promoting glucose transporter-4 vesicle trafficking along cytoskeletal tracks: PAK-Ing them out. Front Endocrinol. 2017;8:329.
Olson AL. Regulation of GLUT4 and insulin-dependent glucose flux. ISRN Mol Biol. 2012;856987.
Dornas WC, de Lima WG, Pedrosa ML, Silva ME. Health implications of high-fructose intake and current research. Adv Nutr. 2015;6(6):729.
Bano F, Wajeeh M, Baig N, Naz H, Akhtar, N, Hajra N. Antiobesity, antihyperlipidemic and hypoglycemic effects of the aqueous extract of Nigella Sativa seeds (Kalongi)[J]. J Biochem Mol Biol. 2009;42(4):136-140.
Kooti W, Hasanzadeh-Noohi Z, Sharafi-Ahvazi N, Asadi-Samani M, Ashtary-Larky D. Phytochemistry, pharmacology, and therapeutic uses of black seed (Nigella sativa). Chinese Journal of Natural Medicines. 2016;14(10):732-745.
Khan AR, Lateef ZNAA, Al Aithan MA, Bu-Khamseen MA, Al Ibrahim I, Khan SA. Factors contributing to non-compliance among diabetics attending primary health centers in the Al Hasa district of Saudi Arabia. Journal of Family and Community Medicine. 2012;19(1):26.
Prabhakar P, Reeta KH, Maulik SK, Dinda AK, Gupta YK. Protective effect of thymoquinone against high-fructose diet-induced metabolic syndrome in rats. European Journal of Nutrition. 2015;54(7): 1117-1127.
Salama RM, Schaalan MF, Ibrahim ME, Khalifa AE, Elkoussi AA. Effectiveness of telmisartan as an adjunct to metformin in treating type II diabetes mellitus in rats. Open Journal of Endocrine and Metabolic Diseases. 2013;3(03):186.
Ezz EA, El-Mahdy AA, Abbas OA. Arab Journal of Nuclear Sciences and Applications. 2016;49:237.
Rani MP, Krishna MS, Padmakumari KP, Raghu KG, Sundaresan A. Zingiber officinale extract exhibits antidiabetic potential via modulating glucose uptake, protein glycation and inhibiting adipocyte differentiation: an in vitro study. Journal of the Science of Food and Agriculture. 2012;92(9):1948-1955.
Singh P, Srivastava S, Singh VB, Sharma P, Singh D. Ginger (Zingiber officinale): A Nobel Herbal Remedy. Int J Curr Microbiol App Sci. 2018;7:4065.
Yang H. Advances in research on lipid-lowering mechanisms of eight medicinal plants. In AIP Conference Proceedings. 2019;2058(1):020007. Li Y, Tran VH, Duke CC, Roufogalis BD, Planta Medica. 2012;78:1549.
Wei CK, Tsai YH, Korinek M, Hung PH, El-Shazly M, Cheng YB, Chang FR. 6-Paradol and 6-shogaol, the pungent compounds of ginger, promote glucose utilization in adipocytes and myotubes, and 6-paradol reduces blood glucose in high-fat diet-fed mice. International Journal of Molecular Sciences. 2017;18(1):168.
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