Toxicological Assessment of a Polyherbal Mixture on Organ Weights and Hormonal Profile in Exposed Wistar Rats

Main Article Content

Godswill J. Udom
John A. Udobang
Daniel N. Obot
Nkechi J. Onyeukwu
Ikanna E. Asuquo
Jude E. Okokon


Hormonal imbalance and hypertrophy of internal organs are sensitive indices of toxicity which may be multifactorial (including exposure to certain foods, drugs and herbal remedies), and are implicated in certain conditions such as infertility amongst others. Dr Iguedo Goko Cleanser® is a polyherbal mixture (PHM) often described by promoters as ‘super effective’ for the treatment of various ailments. The present study was aimed to evaluate the exposure-related effects of PHM on hormonal profile and weight of internal organs in Wistar rats of both gender. Thirty Wistar rats (both genders) randomly allotted to six groups (5 in each group) were exposed orally to PHM for 60 days. Control groups (1 and 4; 10 mL/kg distilled water), groups 2-3 and 5-6 received PHM (476.24; 158.75 mg/kg) respectively. On 62nd day, animals were euthanized under diethyl ether anaesthesia and sacrificed. Blood samples were collected for hormonal analysis and vital organs were eviscerated for macroscopic examinations. PHM significantly (P=.05) decreased spleen weight in female subjects relative to control. However, female rats had higher spleen weights compared to male rats. HDF (high dose females) had increased brain weight relative to control groups. Female rats had dose-dependent increased in progesterone relative to control. Female rats had higher progesterone concentration relative to males. Female rats recorded increased estradiol compared to male rats. However, this was reduced in HDF relative to control; low dose females had elevated estradiol compared to control and HDF. Results of the present day study strongly suggest caution and good judgement as regards the prolonged use of the polyherbal mixture especially as the probable hypertrophy of the brain observed in experimental female rats is not negligible.

Herbal remedy, hypertrophy, hormonal imbalance, toxicity

Article Details

How to Cite
Udom, G. J., Udobang, J. A., Obot, D. N., Onyeukwu, N. J., Asuquo, I. E., & Okokon, J. E. (2020). Toxicological Assessment of a Polyherbal Mixture on Organ Weights and Hormonal Profile in Exposed Wistar Rats. Asian Journal of Research and Reports in Endocrinology, 3(2), 29-36. Retrieved from
Original Research Article


Huizen J. What to know about hormonal imbalances. Available:

Accessed 20 July 2020.

Farah AO, Nooraain H, Noriham A, Azizah AH, Nurul HR. Acute and oral subacute toxicity study of ethanolic extract of Cosmos caudatus leaf in Sprague Dawley rats. Int J Biosci, Biochem & Bioinform. 2013;3(4):301-305.

Ping KY, Darah I, Chen Y, Sreeramanan S. Sasidharan, S. Acute and subchronic toxicity study of Euphorbia hirta L. methanol extract in rats. Biomed Res Int. 2013;2:1-14.

Solaade A. Assorted herbal mixtures: Herbs or poison; 2015.


Accessed 31 March 2019.

Shad KA, Lajbar K, Iqbal H, Khan BM, Akhtar N. Profile of heavy metals in selected medicinal plants. Pakistan J Weed Sci Res. 2008;14:101-110.

Ajasa AMO, Bello MO, Ibrahim AO, Ogunwande IA, Olawore NO. Heavy trace metals and macronutrients status in herbal plants of Nigeria. Food Chem. 2004;85:67-71.

Udom GJ, Okokon JE, Udobang JA, Obot DN, Asuquo IE. Nephrotoxicity assessment of Dr Iguedo Goko Cleanser® in exposed Wistar rats. Asian J Res Med Pharm Sci. 2020;9(1):30-40.

Tanira MOM, Agell AM, Tariq M, Mohsin A, Shah AH. Evaluation of some pharmacological, microbiological and physical properties of Ziziphus spina Christi. Int J Crude Drug Res. 1988;26:56-60.

Yemitan OK, Adeyemi OO. Toxicity studies of the aqueous root extract of Lecaniodiscus cupanioides. Nig J Health Biomed Sci. 2004;3:20-23.

Michael B, Yano B, Sellers RS, Perry R, Morton D, Roome N, et al. Evaluation of organ weights for rodents and non-rodent toxicity studies: A review of regulatory guidelines and a survey of current practices. Toxicol Pathol. 2007;35:742-750.

Swirski FK, Nahrendorf M, Etzrodt M, Wildgruber M, Cortez-Retamozo V, Panizzi P, et al. Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Sci. 2009;325(5940): 612-616.

Amorosa LF. Abbreviated tests of endocrine function. In: Walker HK, Hall WD, Hurst JW editors. Clinical methods: the history, physical and laboratory examinations. 3rd Ed. Boston: Butterworth Publishers; 1990.